Table 1.

Kinetic parameters for KRS determined by using EnzCheck

Enzyme	Km ATP, µM*	HillATP	Km Lys, µM†	Hilll-Lys
PfKRS1	68 ± 3	—	413 ± 37	0.89 ± 0.04
CpKRS	346 ± 128	0.71 ± 0.09	1,045 ± 640	0.49 ± 0.06
HsKRS	2.22 ± 0.44	—	1.92 ± 0.37	—

^*Determined in the presence of saturating concentration of the cosubstrate l-lysine, 5 mM l-lysine for Pf and Cp and 0.075 mM for HsKRS.

^†Determined in the presence of saturating concentration of the cosubstrate ATP, 0.5 mM ATP for Pf, 2 mM for Cp, and 0.1 mM for HsKRS.