Fig. 6.
Founder cells of mixed clones regenerate the injured endometrial epithelium post parturition. (A) Experimental design for tracing YFP-labeled epithelial cells during regeneration of endometrial epithelium postpartum. A single low dosage of tamoxifen (0.01 mg/g body weight) was given to adult Keratin19CreERT2;Rosa26YFP/+ mice at diestrus, then they were mated with adult wild-type male mice for establishment of pregnancy. (B) Representative fluorescent images of uterine tissues from nonpregnant (n = 5) and postpartum mice (n = 4). Uteri were collected on day 3 post parturition for analysis. (Scale bar, 100 μm.) (C) Areal percentage of YFP+ mixed epithelia per cross-section of uterine endometrial epithelia. P value from unpaired t test is provided as ***P < 0.001.