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. 2019 Mar 14;116(14):6914–6923. doi: 10.1073/pnas.1819976116

Fig. 4.

Fig. 4.

Mitochondrial localization of Paraphysomonas proteins evolved from plastid-targeted ancestors. This figure shows constructs for N-terminal regions of Paraphysomonas orthologs of plastid genome-associated proteins (i, glutamyl-tRNA synthetase; ii, glycyl tRNA-synthetase) that localize to the mitochondria, stained with MitoTracker Orange. Both genes have two possible methionine initiator codons (Met1 and Met2) that produce identical localizations. (Scale bars, 10 μm.) Additional GFP images for Paraphysomonas plastid proteins and proteins historically associated with the plastid genome are shown respectively in SI Appendix, Figs. S13 and S16; experimental evidence for the loss of the plastid genome is shown in SI Appendix, Fig. S14; TAiL-PCR alignments of genes that once functioned to supported the plastid genome are shown in SI Appendix, Fig. S15; consensus Bayesian topologies for these genes are shown in SI Appendix, Figs. S17 and S18; and an overview of the evolutionary fates of plastid-genome associated proteins in Paraphysomonas is shown in SI Appendix, Fig. S19.