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. 2019 Mar 20;116(14):7033–7042. doi: 10.1073/pnas.1819534116

Fig. 6.

Fig. 6.

PTPN14 loss reduces the expression of differentiation markers after detachment. Adherent N/Tert-mock and -PTPN14 KO cells were detached by trypsinization and replated in ultralow adherence plates before harvesting 12-h postdetachment, then assayed for markers of differentiation, YAP/TEAD targets, and survival after detachment. (A and B) Gene-expression changes induced by suspension were assayed by qRT-PCR targeting markers of differentiation: KRT16 and IVL (A), and YAP/TEAD targets: CTGF and CYR61 (B). mRNA expression was calculated relative to GAPDH. Bar graphs display the mean ± SD of three independent experiments. (C) Survival after detachment was assessed by replating 1,000 cells from suspension, allowing cells to grow for 5 d, and measuring total viable cell area by Crystal violet staining. Three independent experiments are displayed along with mean ± SD. Statistical significance was determined by ANOVA followed by multiple t tests with the Holm-Šídák family-wise error rate correction (*P < 0.05; **P < 0.01; ***P < 0.001).