Fig. 7.

PTPN14 degradation contributes to high-risk HPV E6/E7 immortalization of primary human keratinocytes. Primary HFK cells were transduced with pairs of retroviruses encoding various HPV E6 and E7 and passaged for up to 75 d. (A) Growth curves from representative immortalization experiment. Population doublings were calculated based upon the number of cells harvested at each passage. Statistical significance was determined from three independent experiments by repeated measures two-way ANOVA. Displayed P values represent the column (cell line) factor (*P < 0.05; **P < 0.01). (B) Bar chart shows mean ± SD of selected passages from growth curves in A.