Fig. 2.

Notch1 signaling is activated in the skin of diabetic animals. (A) Representative images of immunohistochemistry staining for Notch1 ICD (N1ICD; red) and DAPI (blue) in skin from db/db diabetic mice and control mice. (Scale bars: 50 µm.) (B–D) Relative mRNA expression of Notch target genes Hey1, Hes1, or Hes5 and Notch ligands Dll4 and Jagged1 in the skin of diabetic animals [db/db mice (B; n = 10), GK rats (C; n = 5), and STZ-induced diabetic mice (D; n = 3)] and in their nondiabetic control littermates. *P < 0.05 (analyzed using the Student t test). (E) Mouse Notch Signaling Target RT² Profiler PCR Array results comparing the expression of key Notch target genes in skin from db/db mice (n = 5) and controls (n = 4).