Fig. 1.

OR14I1 and PDGRFA are required for HCMV infection of epithelial cells. (A) ARPE-19 cells stably expressing the indicated sgRNAs were infected with TB40E-GFP virus (MOI 3.0). Cells were imaged (10×) for GFP expression (green) as an indicator of viral infection at 2 dpi, and the percent GFP-positive cells was quantified. CON, negative control. (B) Immunoblots of lysates from cells in A. (C) Clonally derived sgOR14I1-expressing ARPE-19 cells were infected with TB40E-GFP virus (MOI 3.0). Cells were imaged (10×) for GFP expression (green) as an indicator of viral infection at 2 dpi, and the GFP-positive cells were quantified. (D) Immunoblots of lysates from cells in C. (E) HEL fibroblasts stably expressing the indicated sgRNAs were infected with AD169 virus (MOI 3.0). Infectivity was determined by cytopathic effect at 2 dpi. (F) Immunoblots of lysates from cells in E. (G) ARPE-19 cells stably expressing the indicated shRNAs were infected with TB40E-GFP virus (MOI 2.0) and then imaged (10×) as in A. (H) ARPE-19 cells stably expressing the indicated shRNAs were infected with TB40E-GFP virus (MOI 2.0). GFP expression was determined at 2 dpi using flow cytometry. The plot depicts GFP versus forward scatter (FSC). (I) Immunoblots of lysates from cells in G. (J) HEL fibroblasts stably expressing the indicated shRNAs were infected with AD169 virus (MOI 2.0). Infectivity was determined by cytopathic effect at 2 dpi. (K) Immunoblots of lysates from cells in J. Actin serves as a loading control throughout. Representative images of three independent experiments are shown. Data represent the mean of n = 3 experiments ±SD. ***P < 0.001, ****P < 0.0001.