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. 2019 Mar 15;116(14):6806–6811. doi: 10.1073/pnas.1818744116

Fig. 2.

Fig. 2.

(A) Exchange-rate constants measured at pH 6.5 (○) and pH 7.4 (●) plotted against the rate constants at pH 6.0 at 25 °C. The solid line indicates the response expected for an EX1 mechanism, and the dotted and dashed lines indicate the responses expected for an EX2 mechanism at pH 6.5 and 7.4, respectively. (B) Exchange-rate constants at pH 7.4 of V31A (●) and E32R (○) are plotted against the rate constants at pH 6.5 at 25 °C. The solid line indicates the response expected for an EX1 mechanism, and the dashed lines indicate the responses expected for an EX2 mechanism at pH 7.4 for the Di-III_14 variants.