NSCLC cell viability and invasion were regulated by GAS5. (A) CCK-8 assay was used in A549 cells transfected with si-NC or si-GAS5 and H522 cells transfected with Vector or GAS5 for NSCLC proliferation. (B) Cell invasion assay was used in A549 cells. (C–E) CCK-8 assay was used to assess cell viability in A549 and H522 cells lines. (C) miR-ctrl or miR-205 was transfected in A549 cell lines, anti-miR-Ctrl, or anti-miR-205 in H522 cells; (D) Vector, GAS5, or together with miR-NC or miR-205 in A549 cell lines; and (E) si-NC, si-GAS5, or combined with anti-miR-NC or anti-miR-205 in H522 cells. (F–H) Cell invasion assay was used to assess invasiveness in A549 and H522 cells. (F) miR-Ctrl or miR-205 was transfected in A549 cell lines, anti-miR-Ctrl, or anti-miR-205 in H522 cells; (G) Vector, GAS5, or together with miR-NC or miR-205 in A549 cells; and (H) si-NC, si-GAS5, or combined with anti-miR-NC or anti-miR-205 in H522 cells. * P<0.05.