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. 2019 Mar 30;25:2311–2319. doi: 10.12659/MSM.912581

Figure 4.

Figure 4

PTEN expression was upregulated by GAS5 via sponging miR-205 in NSCLC cells. (A) The putative wild-type miR-205 binding sites and the mutated miR-205 binding sites in the 3′UTR of PTEN mRNA. (B, C) Luciferase activity was detected by use of luciferase reporter assay in A549 and H522 cells. (B) The A549 cell lines were co-transfected with PTEN-3′UTR-Wt or PTEN-3′UTR-Mut and miR-NC or miR-205. (C) H522 cells with PTEN-3′UTR-Wt or PTEN-3′UTR-Mut and anti-miR-NC or anti-miR-205. (D, F) RT-PCR was employed to quantify the expression of miR-205 in A549 and H522 cells transfected with anti-miR-205 or anti-miR-NC (D) and miR-205 or miR-NC. (E, G–I) Western blot assay was conducted to detect the protein level of PTEN in A549 cells and H522 cells, (E) anti-miR-205 or anti-miR-NC in A549 cells, (G) miR-205 or miR-NC in H522 cells, (H) si-NC, si-GAS5, or co-transfected with anti-miR-NC or anti-miR-205 in A549 cells, and (I) Vector, GAS5, or co-transfected with miR-NC or miR-205 in H522 cells. (J, K) Correlation between miR-205 and PTEN mRNA expression in 6 NSCLC cell lines (J) is negative or (K) positive. *P<0.05.