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. 2019 Apr 3;14:2383–2395. doi: 10.2147/IJN.S196842

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© 2019 Abd Ellah et al. This work is published and licensed by Dove Medical Press Limited

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Flow cytometry detection of regulatory T (T_reg) cells.

Notes: (A) Forward and side scatter histogram depicting lymphocyte population (R1). (B) The expression of CD4 was assessed in the lymphocyte population (R1) and CD4⁺ T cells were gated. (C) Then, the expression of CD25 and FOXP3⁺ cells on CD4⁺ T cells was assessed to detect CD4⁺CD25⁺FOXP3⁺ T_reg cells.

Abbreviations: SSC, side scatter; FSC, forward scatter; FITC, fluorescein isothiocyanate.