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. 2019 Apr 4;12:2519–2529. doi: 10.2147/OTT.S195234

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© 2019 Zhou et al. This work is published and licensed by Dove Medical Press Limited

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SIRT1 promoted HCC cell death.

Notes: HepG2 cells received a supplement of conditioned medium from RAW 264.7 macrophages (MCM), which were transfected with SIRT1 or vector. (A, B) SIRT1 promoted cell death as demonstrated by FC. (C) SIRT1 promoted cell death as demonstrated by TUNEL assays. HepG2 cells received a supplement of conditioned medium from HL-60 macrophages (MCM), which were transfected with SIRT1 or vector. (D, E) Cell death as demonstrated by FC (D) and TUNEL assays (E). Results are displayed as mean ± SEM. **P<0.01 vs vector-MCM group.

Abbreviations: HCC, hepatocellular carcinoma; FC, flow cytometry; MCM, macrophage-conditioned medium; SEM, standard error of mean.