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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Methods Mol Biol. 2018;1844:219–236. doi: 10.1007/978-1-4939-8706-1_15

Fig. 1.

Fig. 1

(a) The 26S proteasome consists of three subcomplexes, the 20S core (gray), the base (blue, containing the AAA+ motor), and the lid (yellow, containing the deubiquitinating enzyme Rpn11 shown in green). (b, c) Maps of the plasmids used for expression of the base (b) and lid (c). Each plasmid was constructed by insertion of the respective proteasome-subunit genes from Saccharomyces cerevisiae S288C into a duet vector (pETDuet, pCOLADuet, pACYCDuet). The majority of genes are expressed with their own T7 promoter (indicated by an arrow at the beginning of the ORF), but there is only one T7 terminator per plasmid