Table 3.
Number of replicates in which assays M1b and M3 detected each target in genomic DNA at the paired-assay limit of detection (LOD; 10 fg B. burgdorferi s.s., 200 fg A. phagocytophilum, 200 fg Ba. microti) in the presence of DNA from a second pathogen at 10–103 x its LOD.
| Second pathogen |
B. burgdorferi s.s. (10 fg) |
A. phagocytophilum (200 fg) |
Ba. microti (200 fg) |
|||
|---|---|---|---|---|---|---|
| fliD (M1b) | 16S (M3) | p44 (M1b) | msp4 (M3) | sa1 (M1b) | 18S (M3) | |
| 2 pg A. phagocytophilum | 9/10 | 10/10 | n/a | n/a | 9/10 | 10/10 |
| 20 pg A. phagocytophilum | 7/10 | 10/10 | n/a | n/a | 7/10 | 10/10 |
| 200 pg A. phagocytophilum | 8/10 | 10/10 | n/a | n/a | 9/10 | 10/10 |
| 2 pg Ba. microti | 9/10 | 10/10 | 10/10 | 10/10 | n/a | n/a |
| 20 pg Ba. microti | 7/10 | 10/10 | 10/10 | 10/10 | n/a | n/a |
| 200 pg Ba. microti | 8/10 | 10/10 | 10/10 | 10/10 | n/a | n/a |
| 100 fg B. burgdorferi s.s. | n/a | n/a | 10/10 | 10/10 | 9/10 | 10/10 |
| 1 pg B. burgdorferi s.s. | n/a | n/a | 10/10 | 9/10 | 8/10 | 10/10 |
| 10 pg B. burgdorferi s.s. | n/a | n/a | 10/10 | 10/10 | 9/10 | 10/10 |