Figure 7: Oxidative stress impairs post-wounding survival in osteocytes.

Osteocytes wounded in the presence of hydrogen peroxide (H₂O₂, 1 mM) demonstrated increased uptake of propidium iodide 5 minutes after wounding, indicative of membrane repair failure and cell death. However, pre-treatment with Vitamin E (220 μM) or Trolox (220 μM, a water-soluble form of Vitamin E) for 24 hours prior to wounding counteracted the negative impact of H₂O₂ on post-wounding cell survival. *p<0.05 vs. Control –H₂O₂ by Tukey’s HSD post-hoc testing. Mean±SE for each group is shown, and data are representative of 3 independent experiments.