RNA was isolated at 30, 90, 150, and 210 minutes (shown in aqua, purple, green, and red, respectively) after infection of M. smegmatis with ZoeJ; reads mapping to forward (For) and reverse (Rev) strands are shown as indicated. A map of the ZoeJ genome is shown below (see Fig. 1 for details). The lysogen RNAseq data is shown for comparison in blue at the top. The positions of several key genes are indicated (9, capsid subunit; 14, major tail subunit; 45, repressor; TMP, Tape Measure Protein).