Fig. 3: The combination treatment suppressed colony formation ability in MB231 and MB453 cells.
(A) MB231 cells were plated into 6-well plates in 2 ml complete media at 1000 cells/well. Cells were treated with or without palbociclib at 250 nM and/or MLN0128 at 31.25 μM for 7 days. MB453 cells were plated at 2000 cells/well and treated with or without palbociclib at 62.5 nM and/or MLN0128 at 7.8125 nM for 21 days. (B) Colony numbers from the four treatment groups were counted. Data are mean values ± SD, from duplicate measurements for each treatment. Statistic analyses were subjected unpaired t test compared to the combination treatment. *p < 0.05, **p < 0.01.