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. 2019 Apr 8;15(4):e8689. doi: 10.15252/msb.20188689

Figure EV1. TRAPP and PAR‐TRAPP techniques and reproducibility.

Figure EV1

  1. Pairwise Pearson correlation coefficients for protein Log2 +UV/−UV ratios obtained in S. cerevisiae with TRAPP and PAR‐TRAPP experiments. Forward isotopic labelling and reverse isotopic labelling are indicated as “FWD” and “RV”, respectively.
  2. Data for Escherichia coli 1 forward and 2 reverse labelling TRAPP repeats were processed as in (A).
  3. The analysis of S. cerevisiae forward and reverse PAR‐TRAPP experiments upon sorbic acid exposure performed as in (A).
  4. Effect of 4tU and UVA treatments on the growth of yeast cells. Exponentially growing yeast cells were treated for 2 h with 4‐thiouracil at the indicated concentrations. The cultures were then irradiated with 350 nm UVA light in the eBox for 30 s delivering 5.8 J cm−2. The lag time of treated cultures was measured by monitoring samples growth curve with Tecan sunrise instrument. Samples: “4tU wash out +UVA”—growth delay of 4‐thiouracil‐treated UVA‐irradiated cells, compared to UVA‐exposed sample. 4tU was removed prior to irradiation; “4tU w/o wash out +UVA”—As sample 1, but 4tU persisted in the media while cells were irradiated; “4tU alone”—growth delay of cells treated with 4tU for 2 h as compared to untreated cells without irradiation.
  5. Frontal view on the eBox irradiation apparatus. The frontal door and the shutters are not present on the picture. Red arrows indicate rails for shutters, designed to prevent sample exposure to UV light, while the lamps are warming up for stable UVA output. The UVA transparent sample tray made of borosilicate glass is placed between the two UVA lamp banks.