Table 2.
Breakdown of cell counting data for the mean percentage/nodose ganglion of neural crest-derived cells (GFP+) that expressed Elavl3/4 (i.e., that were neurons) or Phox2b after vagal neural fold grafts from GFP-transgenic donors. E, embryonic day; n, number; NC, neural crest; Otic, otic placode level; S, somite level; s.d., standard deviation.
| Marker | Elavl3/4 | Phox2b | ||
|---|---|---|---|---|
| Graft type |
Unilateral |
Unilateral | ||
| Region grafted | Otic to S1 | Otic to S6 | Total | Otic to S1a; Otic to S6 |
| Stage analysed | E7.5–E9.5 | E5.5–E9.5 | E5.5–E9.5 | E5.5–E9.5 |
| Mean % NC-derived | 9.6 | 4.3 | 5.8 | 2.7 |
| s.d. | 0.2 | 2.6 | 3.3 | 1.8 |
| n nodose ganglia | 4 | 10 | 14 | 7b |
| n embryos | 4 | 10 | 14 | 6 |
| n cells counted | 2215 | 3852 | 6067 | 1875 |
| Minimum n cells | 310 | 130 | 130 | 130 |
| Maximum n cells | 782 | 1679 | 1679 | 435 |
One E8.5 embryo.
One unilaterally grafted E6.5 embryo had a large contribution of labelled neural crest cells to both nodose ganglia, so both were analysed.