Characterization of lipoprotein contents separated by the acoustofluidic method. (a) VLDL, (b) LDL, and (c) HDL level in plasma sample, sample collected from negative contrast outlet, and sample from positive contrast outlet are tested. Data is obtained from three individual tests using the same plasma sample. *: p < 0.05, **: p < 0.01. (d) Western blot of samples collected from negative contrast outlet, positive contrast outlet and plasma. EV markers (HSP90, TSG101 and CD63) show high expression in positive contrast outlet and plasma, while no expression in negative contrast outlet.