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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Mol Pharm. 2018 Oct 1;15(11):4814–4826. doi: 10.1021/acs.molpharmaceut.8b00434

Figure 12.

Figure 12.

Transfection efficiency of mPEG–OC/DNA nanocomplexes (N/P = 20) in HEK-293 cells. (a1–a3) HEK-293 cells transfected with mPEG–OC/DNA nanocomplexes without nebulization at pH = 6.5, (b1–b3) HEK-293 cells transfected with mPEG–OC/DNA nanocomplexes with nebulization at pH = 6.5, (c1–c3) HEK-293 cells transfected with mPEG–OC/DNA nanocomplexes without nebulization at pH = 6.8, (d1–d3) HEK-293 cells transfected with mPEG–OC/DNA nanocomplexes with nebulization at pH = 6.8, (e1–e3) HEK-293 cells transfected with lipofectamine 3000. (a1–e1) contrast phase images, (b1–e1) fluorescent images, and (c1–e1) flow cytometry dot-plot (SSC-H/GFP-H).