Characterization of mPEG–C and subproducts by using 1H NMR. (a) Medium molecular weight chitosan (C), (b) N-phthaloyl medium molecular weight chitosan (NPHC), (c) mPEG5000, (d) PEG monomethyl ether succinate (mPEG–COOH), (e) PEGylated N-phthaloyl medium molecular weight chitosan (mPEG–NPHC), (f) PEGylated medium molecular weight chitosan (mPEG–C). (c) and (d) are in D2O, (a) and (f) are in 1% DCl/D2O, (b) and (e) are in DMSO.