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. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Biochim Biophys Acta Gen Subj. 2017 May 8;1861(8):1992–2006. doi: 10.1016/j.bbagen.2017.05.006

Fig. 1.

Fig. 1.

NSC145366 interacts with human Hsp90α and specifically binds to the Hsp90 CTD. A) The chemical structure of NSC145366. B) The DARTs assay demonstrating NSC145366 binds to Hsp90α. Hsp90α was incubated with 200 μM of compound and pronase added at the indicated dilution factors (DF). Addition of NSC145366 results in protection of Hsp90α. C) The presence of NSC145366 protects GST-Hsp90α CTD (626–732) from proteolysis in the DARTs assay. KU-32 is a known CTD binder and AUY922 is a NTD binder. D) NSC145366 does not inhibit the proteolysis of GST-Hsp90α NTD (9–236) in the DARTs assay but AUY922 does inhibit proteolysis of the NTD.