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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Regen Eng Transl Med. 2018 Jun 29;5(1):1–9. doi: 10.1007/s40883-018-0068-9

Figure 3. Genetic deletion of CD140b in ASCs impaired direct angiogenic effect on co-cultured HREs.

Figure 3

Representative images of HREs co-cultured with control siRNA treated ASCs or CD140b siRNA treated ASCs for 6 days. (A) Upper panel shows colored images of HREs, stained with Isolectin B4 (red); ASCs, stained for α-SMA (green), and co-cultures counter-stained with DAPI (blue). Lower panel shows Red-only channels representing angiogenic tubes stained with Isolectin B4 (4× magnification). (B) Representative high magnification images of ASCs and HREs co-culture. (C) Image analysis of vascular tube length calculated by image J software as pixels/field. Data represent Mean ± SEM performed in triplicates. *, p<0.05 using unpaired Student T-test; n=3 donors.