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. 2019 Apr 8;14(4):e0206715. doi: 10.1371/journal.pone.0206715

Fig 1. Heat shock induces nuclear foci detectable with dsRNA-specific antibody J2.

Fig 1

Mid-animal intestinal region of 4th larval stage drSI13 worm fixed 40 minutes after heat shock at 35° C. (A) Nuclear J2 foci (red arrows). (B) HSF-1 foci (green arrows). (C) Overlap of J2 foci and HSF-1 foci (orange arrows). White size bar in bottom right corner (20 microns across). DNA stained with DAPI (blue). (D) Quantification of occurrence of HSF-1 and J2 foci over time. 19–20 worms scored per time point with 4 intestinal nuclei scored per worm.