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. 2019 Apr 8;14(4):e0215009. doi: 10.1371/journal.pone.0215009

Table 2. Plasmids used in this study.

Plasmid Descriptions Reference or source
YEplac181 Multi-copy vector carrying LEU2 [38]
YEplac195 Multi-copy vector carrying URA3 [38]
YCplac22 Low copy vector carrying TRP1 [38]
YCplac33 Low copy vector carrying URA3 [38]
YCplac111 Low copy vector carrying LEU2 [38]
YEp181-SFH1 YEplac181 carrying SFH1 This study
YEp181-SFH2 YEplac181 carrying SFH2 This study
YEp181-SFH3 YEplac181 carrying SFH3 This study
YEp181-SFH4 YEplac181 carrying SFH4 This study
YEp181-SFH5 YEplac181 carrying SFH5 This study
YEp181-SEC14 YEplac181 carrying SEC14 This study
YEp195-SFH1 YEplac195 carrying SFH1 This study
YEp181-SFH1S175I,T177I YEplac181 carrying sfh1S175I,T177I This study
YEp181-SFH1R61A,T238D YEplac181 carrying sfh1R61A,T238D This study
YEp181-SFH1L179W,I196W YEplac181 carrying sfh1L179W,I196W This study
YEp181-SFH1Y113C YEplac181 carrying SFH1Y113C This study
YCp111-PSD1 YCplac111 carrying PSD1 This study
YEp181-PSD2 YEplac181 carrying PSD2 This study
YEp181-DPL1 YEplac181 carrying DPL1 This study
YEp181-PSS1 YEplac181 carrying PSS1 This study
YCp33-PSS1-EGFP YCplac33 carrying PSS1-EGFP This study
YCp22-PSD2-FLAG YCplac22 carrying PSD2-FLAG This study
pT-TRP1 Plasmid carrying TRP1 [39]
pT-HIS3 Plasmid carrying HIS3 [39]
pAG32 Plasmid carrying hph [40]
pAG25 Plasmid carrying nat1 [40]
YCpTGAP111 Plasmid carrying GAPDH terminator [41]
pEZZ18 Plasmid carrying ZZ GE healthcare
pEGFP Plasmid carrying EGFP Clontech
pFA6a-3HA-kanMX6 Plasmid carrying 3xHA tag [42]
p3xFLAG-myc-CMVTM-26 Plasmid carrying 3xFLAG tag Sigma
YEp181-SFH1-ZZ YEplac181 carrying SFH1ZZ This study
YEp181-SFH1S175I,T177I-ZZ YEplac181 carrying SFH1S175I,T177IZZ This study
pET28a An expression plasmid Novagen
pETSFH1 A plasmid to express Sfh1 in E. coli This study
pETSFH1S175I,T177I A plasmid to express Sfh1 S175I,T177I in E. coli This study
YEp181-SFH1-HA YEplac181 carrying SFH1-HA This study
YEp181-SFH1-EGFP YEplac181 carrying SFH1-EGFP This study
YEp181-SFH1S175I,T177I-EGFP YEplac181 carrying sfh1S175I,T177I-EGFP This study
YEp181-SFH1R61A,T238D-EGFP YEplac181 carrying sfh1R61A,T238D-EGFP This study
YEp181-SFH1L179W,I196W-EGFP YEplac181 carrying sfh1L179W,I196W-EGFP This study
YCp33-MDM34 YCplac33 carrying MDM34 This study
YCp111-MDM34 YCplac111 carrying MDM34 This study