Skip to main content
. Author manuscript; available in PMC: 2019 Apr 17.
Published in final edited form as: Vaccine. 2019 Mar 25;37(17):2401–2407. doi: 10.1016/j.vaccine.2019.02.007

Figure 4. The C10 motif does not contribute to OspC directed bactericidal Ab responses.

Figure 4.

Antisera were generated against type A OspC and OspCΔ10 in rats and used to screen immunoblots of the recombinant proteins. The left and right images of Panel A present the immunoblot results obtained with anti-OspC and anti-OspCΔC10, respectively. Panel B presents the result of SDS-PAGE and immunoblot analyses of the strains (indicated in the figure) employed in the bactericidal assays. All methods were as detailed in the text. Each panel is labeled to indicate the antiserum used to screen each blot. Note that B31-HE-OspC is a genetically engineered strain of B. burgdorferi B31 that constitutively produces OspC at a high level [51]. Panel C presents the results of bactericidal assays. The rationale for the strains tested and the controls used is presented in the text. The strains tested are indicated above the bar graphs in the figure. Abbreviations are as follows: GPS - guinea pig serum; HI - heat inactivated. All assays were performed in triplicate. The Student’s t-test was used to assess statistical significance (* p < 0.05).