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. 2019 Apr 8;14(4):e0215106. doi: 10.1371/journal.pone.0215106

Fig 1. Purification scheme and analysis of BG505 SOSIP.664 trimers in ExpiCHO-S cultures.

Fig 1

(A) The culture supernatant (red flask) was processed via the affinity columns, in the manners indicated by the arrows, leading to the purified trimer yields recorded below each column. (B) Coomassie blue-stained BN-PAGE analysis of ExpiCHO-S expressed BG505 SOSIP.664 trimer purified by the different affinity columns. The molecular masses of marker (M) proteins (thyroglobulin and ferritin) are indicated.