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. 2019 Apr 8;14(4):e0215106. doi: 10.1371/journal.pone.0215106

Fig 2. Effects of plasmid DNA and transfection components on PGT145 purification.

Fig 2

Aliquots of 2G12/SEC-purified BG505 SOSIP.664 trimers were incubated as indicated and then passed down a PGT145 column, and the yields recorded as a percentage of the initial amount present (300 μg). (A) Incubation in PBS, or with an ExpiCHO-S culture supernatant from non-transfected or mock-transfected cells, or with 500 μg of the DNA expression plasmid as indicated. (B) Incubation in ExpiCHO-S cell culture supernatant, without or with the Feed and Enhancer supplements, as indicated.