Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal anti-V5 |
Invitrogen | Invitrogen:R960-25; RRID:AB_2556564 |
1:2000 |
| Antibody | Mouse monoclonal anti-Pgk1 |
Abcam | Abcam:ab113687; RRID:AB_10861977 |
1:10,000 |
| Antibody | Rabbit polyclonal anti-Rpb1 |
Santa Cruz Biotechnology | Santa Cruz:sc-25758; RRID:AB_655813 |
1:1000 |
| Antibody | Rabbit polyclonal anti-Hsp104 |
Enzo Life Sciences | Enzo:ADI-SPA-1040 | 1:1000 |
| Antibody | Mouse monoclonal anti-Hsp70 |
Abcam | Abcam:ab5439; RRID:AB_304888 |
1:1000 |
| Antibody | Rabbit monoclonal anti-phos-Rps6 (Ser235/236) |
Cell Signaling Technology | Cell Signaling:4858; RRID:AB_916156 |
1:2000 |
| Antibody | Rabbit polyclonal anti-phos-eIF2α (Ser51) |
Invitrogen | Invitrogen:44–728G; RRID:AB_2533736 |
1:1000 |
| Chemical compound, drug |
auxin (indole-3-acetic acid) | Sigma-Aldrich | Sigma-Aldrich:I3750 | |
| Chemical compound, drug |
diazaborine | Millipore Sigma | Millipore Sigma:530729 | |
| Chemical compound, drug |
cycloheximide | Sigma-Aldrich | Sigma-Aldrich:C4859 | |
| Chemical compound, drug |
L-azetidine-2-carboxylic acid | Sigma-Aldrich | Sigma-Aldrich:A0760 | |
| Chemical compound, drug |
diamide | Sigma-Aldrich | Sigma-Aldrich:D3648 | |
| Chemical compound, drug |
rapamycin | LC Labs | LC Labs:R-5000 | |
| Chemical compound, drug |
beta-estradiol | Sigma-Aldrich | Sigma-Aldrich:E2758 |