Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (E. coli) |
S17 λ-pir | de Lorenzo and Timmis, 1994 | Wild type | |
| Strain, strain background (V. cholerae) |
C6706str2 | Thelin and Taylor, 1996 | El Tor wild type | |
| Strain, strain background (V. cholerae) |
JY283 | Yan et al., 2017 |
vpvCW240R ΔpomA
(denoted WT) |
|
| Strain, strain background (V. cholerae) |
JY285 | Yan et al., 2017 | vpvCW240R ΔpomAΔbap1ΔrbmC | |
| Strain, strain background (V. cholerae) |
JY286 | Yan et al., 2017 | vpvCW240R ΔpomAΔrbmAΔbap1ΔrbmC | |
| Strain, strain background (V. cholerae) |
JY287 | Yan et al., 2017 | vpvCW240R ΔpomAΔvpsL | |
| Strain, strain background (V. cholerae) |
JY370 | Yan et al., 2017 | vpvCW240RΔpomA lacZ:Ptac-mKate2:lacZ | |
| Strain, strain background (V. cholerae) |
JY376 | Yan et al., 2017 | vpvCW240RΔpomA ΔvpsL lacZ:Ptac-mKate2:lacZ | |
| Strain, strain background (V. cholerae) |
JY395 | This study |
vpvCW240RΔpomA
Δbap1ΔrbmC lacZ: Ptac-mKate2:lacZ |
|
| Recombinant DNA reagent |
Plasmid: pKAS32 | Skorupski and Taylor, 1996 | Suicide vector, AmpR SmS | |
| Recombinant DNA reagent |
Plasmid: pNUT144 | Drescher et al., 2014 | Suicide vector, AmpR KanR SmS | |
| Recombinant DNA reagent |
Plasmid: pNUT157 | Drescher et al., 2014 | pNUT144 vpvCW240R | |
| Recombinant DNA reagent |
Plasmid: pCMW112 | Hammer and Bassler, 2003 | pKAS32 ΔvpsL | |
| Recombinant DNA reagent |
Plasmid: pCN004 | Nadell and Bassler, 2011 | pKAS32 lacZ:Ptac-mKate2:lacZ | |
| Recombinant DNA reagent |
Plasmid: pCN007 | Nadell et al., 2015 | pKAS32 ΔrbmA | |
| Recombinant DNA reagent |
Plasmid: pCN008 | Nadell et al., 2015 | pKAS32 ΔrbmC | |
| Recombinant DNA reagent |
Plasmid: pCN009 | Yan et al., 2016 | pKAS32 Δbap1 | |
| Recombinant DNA reagent |
Plasmid: pCDN010 | Nadell et al., 2015 | pKAS32 ΔpomA | |
| Software, algorithm | MATLAB and ImageProcessing Toolkit | Mathworks, 2015 | https://www.mathworks.com/products/matlab.html | |
| Software, algorithm | PRISM version 6.07 | GraphPad, 2015 | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | Image composite editor version 2.0.3 | Microsoft, 2015 | https://www.microsoft.com/en-us/research/project/image-composite-editor/ | |
| Software, algorithm | Gmsh version 3.0.6 | Geuzaine and Remacle, 2009 | https://gmsh.info | |
| Software, algorithm | Paraview version 5.5.0 |
Ahrens et al., 2005 | https://www.paraview.org/ | |
| Software, algorithm | FEniCS version 2017.2.0 |
Alnæs et al., 2015 | https://fenicsproject.org/ | |
| Software, algorithm | DigiCamControl software version 2.0.72.0 |
DigiCamControl, 2015 | http://digicamcontrol.com/ | |
| Software, algorithm | Leica Map Start version 7.4.8051 |
Leica, 2017 | https://www.leica-microsystems.com/products/microscope-software/details/product/leica-map/ | |
| Software, algorithm | ImageJ and freehand line selection tool |
NIH | https://imagej.nih.gov/ij/ | |
| Software, algorithm | RheoPlus version 3.40 |
Anton Paar, 2008 | ||
| Other | LB broth, Miller | ThermoFisher | Cat# BP1426-2 | |
| Other | Bacto agar | VWR | Cat# 214030 | |
| Other | O.C.T. agent | Tissue-Tek, Sakura | Cat# 4583 | |
| Other | Silicone oil, 5 cSt | Sigma Aldrich | Cat# 317667 | |
| Other | Glass beads, acid washed, 425 – 600 µm diameter |
Sigma Aldrich | Cat# G8772 | |
| Other | MP Biomedicals Roll & Grow Plating Beads, 4 mm in diameter | ThermoFisher | Cat# MP115000550 | |
| Other | BD PrecisionGlide needles (0.6 mm × 2.5 mm) |
Sigma Aldrich | Cat# Z118044 | |
| Other | EMD Millipore,25 mm in diameter | Sigma Aldrich | Cat# VSWP02500 | |
| Other | SytoX Green Nucleic Acid Stain |
ThermoFisher | Cat# S7020 | |
| Other | Wheat Germ Agglutinin Sampler Kit |
ThermoFisher | Cat# W7024 | |
| Other | Higgins Black India Ink | |||
| Other | Physica MCR 301 shear rheometer |
Anton Paar, 2008 | ||
| Other | Nikon D3300 SLR digital camera with DX Zoom-Nikkor 18-55 mm lens |
Amazon | https://www.amazon.com/Nikon-1532-18-55mm-3-5-5-6G-Focus-S/dp/B00HQ4W1QE/ref=sr_1_3?ie=UTF8&qid=1492108083&sr=8-3&keywords=D3300&th=1 | |
| Other | Huion L4S light box | Amazon | https://www.amazon.com/Huion-L4S-Light-Box-Illumination/dp/B00J0UUHPO | |
| Other | Sigma 105 mm macro lens for Nikon DSLR camera |
Amazon | https://www.amazon.com/Sigma-258306-105mm-Macro-Camera/dp/B0058NYW3K/ref=sr_1_sc_3?ie=UTF8&qid=1485483491&sr=8-3-spell&keywords=sigma+macroles | |
| Other | Leica stereoscope model M205 FA |
Leica | ||
| Other | Leica DCM 3D micro-optical system |
Leica | https://www.leica-microsystems.com/products/light-microscopes/upright-microscopes/details/product/leica-dcm-3d/ | |
| Other | VR3200 wide-area 3D measurement system |
Keyence | https://www.keyence.com/products/measure-sys/3d-measure/vr-3000/models/vr-3200/index.jsp | |
| Other | FEI XL 30 FEG-SEM |
FEI | https://iac.princeton.edu/equipment.html | |
| Other | Millrock Technology, BT85A-A | Millrock | https://www.millrocktech.com/ | |
| Other | VCR IBS/TM200S ion beam sputterer |
VCR | https://iac.princeton.edu/equipment.html |