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. 2019 Mar 11;8:e43630. doi: 10.7554/eLife.43630

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© 2019, Padavannil et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Author response image 1. — A. SEC profiles of 1) Imp9 alone (blue trace), 2) a previously purified Imp9•RanGTP complex (light blue trace) and 3) a previously purified Imp9•H2A-H2B + excess RanGTP (green; the peak at ~ 16 ml is excess RanGTP). Elution volume for each of the Imp9-containing peaks is listed. B. Fractions for the Imp9 containing peak in the SEC of Imp9•H2A-H2B + excess RanGTP (green trace in A) were pooled and subjected to a second round of SEC to produce the SAXS sample for the heterotetrameric complex.