Skip to main content
. Author manuscript; available in PMC: 2019 Apr 8.
Published in final edited form as: Nat Protoc. 2014 May 1;9(6):1229–1244. doi: 10.1038/nprot.2014.100

Figure 2: Development of the definitive ectoderm epithelium.

Figure 2:

a-c, DIC images of representative aggregates on days 1, 2, and 3 of 3D culture. d-i, Following Matrigel addition on day 1, Laminin is incorporated into a basement membrane (e, f, h, i) on the surface of the aggregate. An epithelium develops on the aggregate surface by day 3. See Table 1 for a list of antibodies used for characterization. ESC, embryonic stem cells. Scale bars, 100 μm