KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Sheep anti-GFP | Biorad | Cat # 4745-1051; RRID: AB_619712 |
| Rat anti-RFP | Chromotek | Cat # 5f8-100; RRID: AB_2336064 |
| Chicken anti-β-galactosidase | Abcam | Cat # 9361; RRID: AB_307210 |
| Rabbit anti-Fos | EMD Millipore | Cat # PC38; RRID: AB_2106755 |
| Donkey anti-sheep, Alexa 488 conjugate | Jackson ImmunoResearch | Cat # 713-545-147; RRID: AB_2340745 |
| Donkey anti-rat, Cy3 conjugate | Jackson ImmunoResearch | Cat # 712-165-150; RRID: AB_2340666 |
| Donkey anti-chicken, Alexa 488 conjugate | Jackson ImmunoResearch | Cat # 703-545-155; RRID: AB_2340375 |
| Donkey anti-chicken, Cy3 conjugate | Jackson ImmunoResearch | Cat # 703-165-155; RRID: AB_2340363 |
| Donkey anti-chicken, Alexa 647 conjugate | Jackson ImmunoResearch | Cat # 703-605-155; RRID: AB_2340379 |
| Donkey anti-rabbit, Cy3 conjugate | Jackson ImmunoResearch | Cat # 711-165-152; RRID: AB_2307443 |
| Bacterial and Virus Strains | ||
| Recombinant adeno-associated virus: AAV1-Syn-flex-GCaMP6s |
Penn Vector Core | Addgene number: 100845 |
| Recombinant adeno-associated virus: AAV1-EF1α-flex-hChR2(H134R):eYFP |
Penn Vector Core | Addgene number: 20298 |
| Recombinant adeno-associated virus: AAV1-EF1α-flex-eNpHR3.0:eYFP |
Penn Vector Core | Addgene number: 26966 |
| Recombinant adeno-associated virus: AAV1-CAG-flex-eGFP |
Penn Vector Core | Addgene number: 59331 |
| Recombinant adeno-associated virus: AAV1-EF1α-taCasp3-TEVp |
UNC Vector Core | Addgene number: 45580 |
| Recombinant adeno-associated virus: AAV1-EF1α-flex-hM4DGi:mCherry |
UNC Vector Core | Addgene number: 50461 |
| Recombinant adeno-associated virus: AAV5-EF1α-flex-mCherry |
UNC Vector Core | Addgene number: 50462 |
| Recombinant adeno-associated virus: AAV1-EF1α-flex-eYFP |
UNC Vector Core | Addgene number: 27056 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DAPI | Sigma-Aldrich | Cat # D9542; CAS 28718-90-3 |
| Clozapine-N-oxide (CNO) | Enzo Life Sciences | Cat # BML-NS105-0005 |
| Estradiol | Sigma-Aldrich | Cat # E8875;CAS 50-28-2 |
| Progesterone | Sigma-Aldrich | Cat # P0130; CAS 57-83-0 |
| 2-phenylethylamine (PEA) | Sigma-Aldrich | Cat # 128945; CAS 64-04-0 |
| Cadaverine | Sigma-Aldrich | Cat # D22606; CAS 462-94-2 |
| Critical Commercial Assays | ||
| Testosterone ELISA kit | Cayman | Cat # 582701 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | Cat # 000664; RRID: IMSR_JAX:000664 |
| Mouse: C57BL/6N | Charles River | Cat # 027 |
| Mouse: Swiss Webster | Charles River | Cat # 024 |
| Mouse: 129/SvEvTac | Taconic Biosciences | Cat # 129SVE-M |
| Mouse: AroCre | Unger et al., 2015 | Cat # 027038; RRID: IMSR_JAX:027038 |
| Mouse: AroIPIN | Wu et al., 2009 | Cat # 012377; RRID: IMSR_JAX:012377 |
| Mouse: Trpc2−/− | Leypold et al., 2002 | N/A |
| Mouse: VgatCre | Vong et al., 2011 | Cat # 028862; RRID: IMSR_JAX:028862 |
| Mouse: Gad1EGFP | Tamamaki et al., 2003 | N/A |
| Software and Algorithms | ||
| ImageJ | NIH |
https://imagej.nih.gov/ij/index.html; RRID: SCR_003070 |
| MATLAB | MathWorks |
https://www.mathworks.com/products.html; RRID: SCR_001622 |
| GraphPad Prism 6 | GraphPad Software |
https://www.graphpad.com/scientificsoftware/prism/; RRID: SCR_002798 |