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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Antiviral Res. 2019 Mar 19;165:47–54. doi: 10.1016/j.antiviral.2019.03.006

Figure 7. The effect of individual core mutants from GYF strain on WT HBV replication.

Figure 7.

HepG2 cells were transfected with 1.6 μg of pCMVHBV-WT (lane 1), pCMVHBV-GYF (lane 2), and pCMVHBV-WT with indicated single HBcAg amino acid mutations from the GYF strain (lane 3–6). Cells and supernatant were harvested at day 5 post-transfection for analyses of total intracellular viral RNA (A), cytoplasmic capsid (B), encapsidated pgRNA (C), core DNA (D), and supernatant viral particles (E). The relative levels of encapsidated pgRNA and core DNA in each sample are expressed as the percentage of RNA and DNA level in the indicated control samples.