Skip to main content
. Author manuscript; available in PMC: 2019 Dec 5.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Nov 19;10(48):41215–41223. doi: 10.1021/acsami.8b18157

Table 1.

Restructuring Yields.a

Restructured Linearf Otherg Total
0 Tailsb 1 Tailc 2 Tailsd Combinede
With Strap Number of
Molecules
50 112 78 240 212 86 538
Percentage of
Molecules
9.3% 21.1% 14.7% 45.1% 38.9% 16.0%
No Strap Number of
Molecules
2 4 2 8 66 14 88
Percentage of
Molecules
2.3% 4.5% 2.3% 9.1% 75.0% 15.9%
a

Number of molecules and corresponding percentages determined to be restructured by an image-based assay of the experimental condition (with strap) and control (no strap; see SI Figure S11 for scheme).

b

0-tailed structures appear as a λ circle.

c

1-tailed structures look like a lollipop.

d

2-tailed structures are bolos as described in the text.

e

”Combined” column is a sum of all of the restructured molecules with 0, 1 or 2 tails.

f

Linear molecules are nodal λ DNA molecules that were not restructured, either with or without tails.

g

“Other” molecules were molecules that could not be classified in any of the other groups, including intermolecular strapping events (SI Figure S12) and nodal λ DNA molecules that had more than one T7-BanII tail attached to a single node.