Table 1.
Restructuring Yields.a
| Restructured | Linearf | Otherg | Total | |||||
|---|---|---|---|---|---|---|---|---|
| 0 Tailsb | 1 Tailc | 2 Tailsd | Combinede | |||||
| With Strap | Number of Molecules |
50 | 112 | 78 | 240 | 212 | 86 | 538 |
| Percentage of Molecules |
9.3% | 21.1% | 14.7% | 45.1% | 38.9% | 16.0% | ||
| No Strap | Number of Molecules |
2 | 4 | 2 | 8 | 66 | 14 | 88 |
| Percentage of Molecules |
2.3% | 4.5% | 2.3% | 9.1% | 75.0% | 15.9% | ||
Number of molecules and corresponding percentages determined to be restructured by an image-based assay of the experimental condition (with strap) and control (no strap; see SI Figure S11 for scheme).
0-tailed structures appear as a λ circle.
1-tailed structures look like a lollipop.
2-tailed structures are bolos as described in the text.
”Combined” column is a sum of all of the restructured molecules with 0, 1 or 2 tails.
Linear molecules are nodal λ DNA molecules that were not restructured, either with or without tails.
“Other” molecules were molecules that could not be classified in any of the other groups, including intermolecular strapping events (SI Figure S12) and nodal λ DNA molecules that had more than one T7-BanII tail attached to a single node.