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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Cytotherapy. 2018 Dec 1;21(3):307–314. doi: 10.1016/j.jcyt.2018.11.004

Figure 2.

Figure 2.

Pre-REP TIL expansion in tissue culture plates with and without 4-1BB. Equal numbers of 48 melanoma tumor fragments from three separate patients were plated in tissue culture wells in IL-2 with or without 4-1BB. The addition of 4-1BB consistently increased the number of tumor fragments with TIL outgrowth (A) and the total number of viable TIL (B). (C) TIL harvested at day 14 were cultured in complete media alone, with melanoma cell lines mismatched or matched for MHC class I antigens or with autologous tumor digests. Culture supernatants were tested for IFN-γ levels by ELISA. The presence of 4-1BB augmented IFN-γ production in 2 of three samples tested. (D) The percent CD8+ cells was increased with 4-1BB in 2 or three samples tested.