Figure 3.

Unc5c Is Expressed in Ventral-Central Retina

(A–E) In situ hybridization (ISH) for Unc5c in coronal retinal sections at E13.5 (A), E14.5 (B), E16.5 (C), E18.5 (D), and P2 (E) mice.

(F) Immunoblot detection of Unc5c and Dcc in control (CT) HEK cells and HEK transfected with Unc5c encoding plasmids and in E13.5–P2 retinal lysates. β-actin was used as a loading control. Graphs show levels of Unc5c and Dcc normalized to actin levels. Peak of Unc5c expression is at E14.5.

(G) Representative images of Unc5c expression (red) in coronal (upper) and horizontal (bottom) retinal sections from E16.5 mouse embryos stained with DAPI (gray).

(H) Mean (± SEM) fluorescent intensity of Unc5c in each quadrant of E16.5 retinas.

(I) Diagram of a whole-mounted retina summarizing the expression of Unc5c (red).

Error bars indicate ± SEM.

See also Figure S2.