REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal antibody anti-β-actin (1:10000 for WB)	Sigma-Aldrich	Cat#A5441; RRID:AB_476744
Goat polyclonal antibody anti-DCC (A-20) (1:100 for WB)	Santa Cruz	Cat#sc-6535; RRID:AB_2245770
Rabbit anti-human Unc5c polyclonal antibody (1:500 for WB)	Abcam	Cat#ab91408; RRID:AB_2212873
Chicken polyclonal antibody anti-GFP (1:2000 for IF)	Aves Labs	Cat#GFP-1020; RRID:AB_10000240
Rabbit polyclonal antibody anti-Zic2 (1:1000 for IF)	This lab (previous study [55, 56, 57, 58])	N/A
Mouse neuron class III β-Tubulin monoclonal antibody (anti-Tuj1) (1:1000 for IF)	Covance	Cat#MMS-435P; RRID:AB_2313773
Rabbit specific beta III tubulin antibody (1:1000 for IF)	Abcam	Cat#ab18207; RRID:AB_444319
Mouse monoclonal antibody anti-Brn3a (1:300 for IF)	Millipore	Cat#MAB1585; RRID:AB_94166
Rabbit polyclonal antibody anti-calbindin D-28k (1:1000 for IF)	Swant	Cat#CB38; RRID:AB_2721225
Goat polyclonal antibody anti-ChAT (1:100 for IF)	Millipore	Cat#AB144P; RRID:AB_2079751
Mouse monoclonal antibody anti-Isl1/2 (39.4D5) (1:500 for IF)	Hybridoma Bank	Cat#39.4D5; RRID:AB_2314683
Bacterial and Virus Strains
pAAV-CAG-tdTomato	Addgene	Cat#59462-AAV5
Chemicals, Peptides, and Recombinant Proteins
Cholera toxin B subunit (CTB)-Alexa 594	ThermoFisher	Cat#C22842
Cholera toxin B subunit (CTB)-Alexa 488	ThermoFisher	Cat#C34775
Cholera toxin B subunit (CTB)-Alexa 647	ThermoFisher	Cat#C34778
TEMED	Sigma-Aldrich	Cat#T9281
Acrylamide solution	Sigma-Aldrich	Cat#A4058
Lipofectamine 2000	Thermo Fisher Scientific	Cat#11668019
Pencillin/Streptomycin	Sigma-Aldrich	Cat#P0781
cOmplete Mini EDTA-free protease inhibitor cocktail tablets	Sigma-Aldrich	Cat#11836170001
IGEPAL CA-630	Sigma-Aldrich	Cat#I8896
Laemmliś buffer	Sigma-Aldrich	Cat#S3401
Protein Assay Dye Reagent Concentrate	Bio-Rad	Cat#5000006
Dimethylsulphoxide (DMSO)	Sigma-Aldrich	Cat#D2650
Paraformaldehyde	Sigma-Aldrich	Cat#30525-89-4
Experimental Models: Cell Lines
HEK293 cells	ATCC	Cat#300192/p777_HEK293; RRID:CVCL_0045
Experimental Models: Organisms/Strains
DBA2	Charles River	N/A
C57BL/6	Charles River	N/A
B6D2F1 (DBA2xC57BL/6)	Instituto de Neurociencias de Alicante, Spain	N/A
Zic2kd/kd (B6.129S4-Zic2tm1Jaru/JaruRbrc Mus musculus)	RIKEN Repository	IMSR Cat#RBRC00165; RRID:IMSR_RBRC00165
Unc5crcm(B6C3Fe a/a-Unc5crcm/J Mus musculus)	Jackson Laboratories	IMSR Cat#JAX:001607; RRID:IMSR_JAX:001607
Pigmented ferrets (Mustela putorius furo)	Euroferret	N/A
Danio Renio (strain AB)	Instituto de Neurociencias de Alicante, Spain	N/A
Gallus gallus, fertilized chicken eggs	Granja Santa Isabel (Córdoba, Spain)	http://www.granjasantaisabel.com; Cat#800008
Oligonucleotides
shRNA primer1: 5′-GATCCGAACCACCGTGACTTTGAGTT CAAGAGACTCAAAGTCACGGTGGTTCTTTTTTGGAA-3′	This paper	N/A
shRNA primer 2: 5′-AGCTTTTCCAAAAAAGAACCACCGTG ACTTTGAGTCTCTTGAACTCAAAGTCACGGTGGTTCG-3′.	This paper	N/A
mUnc5c primer 1: 5′-CGGCCCCGAAGAATGGAGGC-3′	This paper	N/A
mUnc5c primer 2: 5′-GGTCAGCACAACGGGTCGGG-3′	This paper	N/A
zUnc5c primer 1: 5′-GACACGCAGGACGCACTCAAG-3′	This paper	N/A
zUnc5c primer 2: 5′-CCCACAGGTCCAGGATCACTC-5	This paper	N/A
cUnc5c primer 1: 5′-CGGCCCCGAAGAATGGAGGC-3′	This paper	N/A
cUnc5c primer 2: 5′-GGTCAGCACAACGGGTCGGG-3′	This paper	N/A
Recombinant DNA
mNetrin-1 pBluescript	Gift from Prof. Orly RinerW (Weizmann Institute of Science, Israel)	N/A
pCAG-mUnc5c-IresEGFP	This paper	NC_0000069.6
TOPO plasmid	Thermo Fisher Scientific	Cat#451641
pCAG-GFP	This lab (previous study [59])	N/A
pSilencer2.1	Thermo Fisher Scientific	Cat#AM5760M
Software and Algorithms
IMARIS 9.2.1	Bitplane, Zurich, Switzerland	http://www.bitplane.com
Fiji	ImageJ	https://fiji.sc
MATLAB	MathWorks	https://www.mathworks.com; https://github.com/SalvadorSala/SOM
Other
DAPI staining	Sigma-Aldrich	Cat#10236276001
DiI crystals	Thermo Fisher Scientific	Cat#D3911