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. Author manuscript; available in PMC: 2019 Apr 9.
Published in final edited form as: J Microbiol Methods. 2013 Dec 25;98:23–25. doi: 10.1016/j.mimet.2013.11.014

Figure 1. Schematic of Recombination.

Figure 1

The successful single stranded oligonucleotide used was 60 base pairs in length and identical in sequence to a portion of the wild type parC gene, save for the point mutation indicated. The strains integrated the oligo into their genomes at the site of similarity within the parC gene.