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. 2019 Apr 8;9:5757. doi: 10.1038/s41598-019-42077-1

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© The Author(s) 2019, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of the surface displacement profiles for the different cells. The red line is the profile along the apical cap fibers and/or the main cell axis, the blue line is the profile perpendicular to it. The number near the profile is the calculated degree of anisotropy (D.A.). Surface displacement profiles for NIH 3T3 fibroblasts with a well-developed actin cap (a–c), without actin cap (d), after Latrunculin A (LatA) treatment (e) and typical for MDA-MB-231 cell (f) are presented. In (a), the microsphere used as an AFM probe is shown with green for the reference (it has an elliptical shape due to different x-y scaling), and two widths measured at half-depth for calculation of the D.A. are shown with arrows.