Fig. 5. Ion and λ-DNA translocation through an SU-8 nanopore membrane.

a Current versus applied voltage for two nanopores (a 12 nm pore fabricated via NIL (Fig. 2a) and a 6 nm pore via the combination of NIL and the post-NIL polymer reflow process (Fig. 2d)) in a buffer electrolyte. b, c Transient current traces showing the translocation of 48.5 kb λ-DNA through two different nanopores at an applied voltage of 100 mV in the same buffer solution. d The corresponding event density plot constructed from 436 and 126 translocation events at a 100 mV applied bias. e The current blockade histogram and f the translocation duration histogram at 100 mV. NIL, nanoimprint lithography