Fig. 10.

Hepatic RGS16 reconstitution reverses Arg2-mediated improvements in hepatic fat accumulation. a H&E and Oil Red O-stained liver sections from db/db AAV8-Control and AAV8-Arg2 mice treated with Ad-GFP or Ad-RGS16. Scale bar, 100 µm. b Hepatic triglycerides, cholesterol, non-esterified fatty acids and LDL-C in livers from db/db AAV8-Control and AAV8-Arg2 mice treated with Ad-GFP or Ad-RGS16. c Serum ALT, AST and albumin in db/db AAV8-Arg2 mice expressing Ad-GFP or Ad-RGS16. d Hepatic inflammatory gene marker mRNA by qPCR. e mRNA expression of insulin-responsive genes by qPCR (n = 8 mice per group). For box plots, the midline represents the median, boxes represent the interquartile range and whiskers show the full range of values. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student’s t-test