Fig. 3.

Hepatic Arg2 protects against hepatic steatosis and inflammation. a Representative hematoxylin and eosin (H&E)-stained (top) and Oil Red O-stained (bottom) liver sections from AAV8-Control (left) and AAV8-Arg2 (right) mice that were fed NCD or HFrD for 5 weeks. Scale bar, 100 µm. b Triglyceride, cholesterol, non-esterified fatty acid, and LDL-C contents in liver of AAV8-Control or AAV8-Arg2 injected WT mice that fed NCD (n = 6 to 7 mice per group) or HFrD (n = 8 mice per group). c Hepatic expression of genes involved in cytokine and chemokine inflammatory responses. Gene expression was normalized to 36B4 mRNA levels. For box plots, the midline represents the median, boxes represent the interquartile range, and whiskers show the full range of values. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001 relative to vehicle treatment, by two-tailed Student’s t-test