Fig. 9.

Hepatic RGS16 reconstitution reverses Arg2-mediated improvements in peripheral fat accumulation and glucose homeostasis. a Experimental schematic used to test the role of AAV8-mediated mouse Arg2 and Ad-RGS16 overexpression in db/db mice. b Body weight and c body composition of AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). d VO₂, VCO₂, energy expenditure, and RER during light and dark cycles in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). e Serum insulin (left), glucose (middle), and HOMA2 IR (right) in AAV8-Control or AAV8-Arg2 db/db mice expressing Ad-Control or Ad-RGS16 (n = 8 per group). f Intraperitoneal GTT and ITT (n = 5 mice per group). g Western blot analysis of total and phosphorylated AKT in liver samples from db/db AAV8-Control and AAV8-Arg2 mice (n = 4 mice per group). For box plots, the midline represents the median, boxes represent the interquartile range and whiskers show the full range of values. For bar graphs, data represent mean+s.e.m. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.0001; n.s., not significant; relative to control treatment, by two-tailed Student’s t-test