Fig. 2.

RNA-seq of RBFOXi transfected U87MG cells. a RT-PCR of RBFOX1/2 targets in U87MG cells transfected with 2.5 µM of either RBFOXi or SCRM harvested at the indicated time points. b Venn diagram showing the overlap of exons identified as having altered splicing in RBFOX knockdown cells (green) (as previously reported by Damianov et al. ²¹) and our RBFOXi treated cells (purple). Number of FRBOX1/2 motifs (UGCAUG) found in the introns downstream of the alternatively spliced exons in each study are shown above the Venn diagram. c Scatter plot showing the correlation of change in splicing (delta PSI) of the 49 significantly alternatively spliced exons detected in both studies (b) (FDR < 0.05)