Fig. 4.

RBFOXi affects alternative splicing and muscle development in zebrafish. a Scheme of experimental design, where either 5pg or 8pg of RBFOXi (or SCRM) were injected to fertilized zebrafish eggs. b RT-PCR of known splicing targets of RBFOX1/2 in 48 h old larva, after injection with 8pg of either SCRM or RBFOXi. RNA was extracted and reverse transcribed from each individual larva. Gel image of a representative experiment is shown above each column. The same EF1α control is shown for all panels, as the gels are from the same experiment **p-value < 0.003, calculated using Student's t-test (two-tailed). Data represent means ± SD of ten zebrafish samples. c Quantification of phenotype severity 48 h post fertilization after injection with either SCRM or RBFOXi (5pg, 8pg). Phenotypes were assigned by visualization of fiber formation and segmental structures. d Pictures of phalloidin staining of F-actin fibers in fish tails 48 h post fertilization after injection with 8pg of either SCRM or RBFOXi