Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 8;10:1590. doi: 10.1038/s41467-019-09523-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — PTBP1 decoy oligonucleotides inhibit its splicing and biological activities in breast cancer cells. a Western blot of proteins pulled down with biotin conjugated PTBP1i (biPTBP1i) or SCRM (biSCRM) using HEK293 nuclear extracts. b Volcano plot showing statistically significant proteins pulled down with biotin conjugated PTBP1i, compared to SCRM control, using HEK293 nuclear extracts. c RT-PCR and quantification of known PTBP1 splicing targets in MDA-MB-231 cells 48 h after transfection with either 5 µM biSCRM or biPTBP1 oligonucleotides. **p-value <0.0008. Data represent means ± SD of five independent biological samples. d Proliferation assay of MDA-MB-231 cells transfected as in c. Four hours after transfection 4000 cells/well were seeded in six replicates. **p-value <0.0002 for all time points. Data represent means ± SD of six replicates. e Clonogenic assay of MDA-MB-231 cells transfected as in c plated at different densities (250, 500, or 1000 cells/well). n = 2. Data represent means ± SD of duplicate wells. f Soft agar colony growth assay on MDA-MB-231 cells transfected as in c. Graphs represent quantification of 10 fields counted in duplicate (total of 20 fields). **p-value <1.7E−27. Data represent means ± SD of two wells. p-values were calculated using Student's t-test (two-tailed)