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Zinc doped copper oxide nanocomposites induced autophagy via AMPK/mTOR pathway. The level of AMPK, p-AMPK, mTOR, p-mTOR, ULK1, p-ULK1, Beclin-1, and LC3-II/LC3-I were detected by Western Blot in AsPC-1 (A) and MIA PaCa-2 (B) cancer cells. (C,D) represented the quantitative results of relative autophagic gene expression (t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).
