Skip to main content
. 2019 Apr 2;10:667. doi: 10.3389/fimmu.2019.00667

Figure 1.

Figure 1

SATB1 alternative promoter usage during T-helper 2 (Th2) cell differentiation. (A) SATB1 isoforms in CD4+ T-cells identified by RNA-Seq analysis. Three mRNA isoforms of SATB1 with alternative first exons (E1a, E1b, and E1c) that correspond to the usage of three alternative promoters (P1, P2, and P3, respectively). (B) Schematic of in vitro differentiation of naïve CD4+ T-cells into Th2 cells. Naive CD4+ cells were treated with anti-CD3 and anti-CD28 along with IL4 for 96 h to induce Th2 differentiation. (C) Immunoblot assay performed as mentioned in methods using the antibody against GATA3, phospho-STAT6 (pSTAT6), total STAT6 and SATB1 for naïve CD4+ and Th2 cells. Increase in expression of GATA3, pSTAT6, SATB1 confirms the differentiation of naive CD4+ cells into Th2 cells. (D,E) qRT-PCR analysis for total SATB1 expression and SATB1 alternative promoter usage in naive and differentiated Th2 cells. A significant increase in expression of SATB1 is observed which corresponds to increased usage of SATB1 P2 and P3 promoter. Error bars indicate SEM. (N = 6; * < 0.05, ** < 0.01); P-values were calculated using Student's t-test.